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Product Detail
DMEM/F12 Medium
Price :
$11.30
Catalog Number : B1121-038
Detail
Description
COA
User Guide
Formula
Catalog NumberB1121-038
With

L-glutamine, Phenol Red,

HEPES,Sodium Pyruvate

AppearanceClear reddish solution
Product Size500ML
PH7.4
Unit-Price($)11.3
Triple 0.1µm sterile-filtered
Storage conditions2°C to 8°C (protect from light)
Shipping conditionsRoom Temperature
Shelf life12 months from date of manufacture


DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. BIOEXPLORER®offer a variety of DMEM/F-12 modifications for a range of cell culture applications. DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.






DMEM/F-12, HEPES    B1121-038

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

Glycine

75.0

18.75

0.25

L-Alanine

89.0

4.5

0.049999997

L-Arginine   hydrochloride

211.0

147.5

0.69905216

L-Asparagine-H2O

150.0

7.5

0.05

L-Aspartic   acid

133.0

6.65

0.05

L-Cysteine   hydrochloride-H2O

176.0

17.56

0.09977272

L-Cystine   2HCl

313.0

31.29

0.09996805

L-Glutamic   Acid

147.0

7.35

0.05

L-Glutamine

146.0

365.0

2.5

L-Histidine   hydrochloride-H2O

210.0

31.48

0.14990476

L-Isoleucine

131.0

54.47

0.41580153

L-Leucine

131.0

59.05

0.45076334

L-Lysine   hydrochloride

183.0

91.25

0.4986339

L-Methionine

149.0

17.24

0.11570469

L-Phenylalanine

165.0

35.48

0.2150303

L-Proline

115.0

17.25

0.15

L-Serine

105.0

26.25

0.25

L-Threonine

119.0

53.45

0.44915968

L-Tryptophan

204.0

9.02

0.04421569

L-Tyrosine   disodium salt dihydrate

261.0

55.79

0.21375479

L-Valine

117.0

52.85

0.4517094

Vitamins

Biotin

244.0

0.0035

1.4344263E-5

Choline   chloride

140.0

8.98

0.06414285

D-Calcium   pantothenate

477.0

2.24

0.0046960167

Folic   Acid

441.0

2.65

0.0060090707

Niacinamide

122.0

2.02

0.016557377

Pyridoxine   hydrochloride

206.0

2.0

0.009708738

Riboflavin

376.0

0.219

5.824468E-4

Thiamine   hydrochloride

337.0

2.17

0.0064391694

Vitamin   B12

1355.0

0.68

5.0184503E-4

i-Inositol

180.0

12.6

0.07

Inorganic   Salts

Calcium   Chloride (CaCl2) (anhyd.)

111.0

116.6

1.0504504

Cupric   sulfate (CuSO4-5H2O)

250.0

0.0013

5.2E-6

Ferric   Nitrate (Fe(NO3)3"9H2O)

404.0

0.05

1.2376238E-4

Ferrous   Sulfate (FeSO4 7H2O)

278.0

0.417

0.0015

Magnesium   Chloride (anhydrous)

95.0

28.64

0.30147368

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0

48.84

0.407

Potassium   Chloride (KCl)

75.0

311.8

4.1573334

Sodium   Bicarbonate (NaHCO3)

84.0

1200.0

14.285714

Sodium   Chloride (NaCl)

58.0

6995.5

120.61207

Sodium   Phosphate dibasic (Na2HPO4) anhydrous

142.0

71.02

0.50014085

Sodium   Phosphate monobasic (NaH2PO4-H2O)

138.0

62.5

0.45289856

Zinc   sulfate (ZnSO4-7H2O)

288.0

0.432

0.0015

Other   Components

D-Glucose   (Dextrose)

180.0

3151.0

17.505556

HEPES

238.0

3574.5

15.018908

Hypoxanthine   Na

159.0

2.39

0.015031448

Linoleic   Acid

280.0

0.042

1.4999999E-4

Lipoic   Acid

206.0

0.105

5.097087E-4

Phenol   Red

376.4

8.1

0.021519661

Putrescine   2HCl

161.0

0.081

5.031056E-4

Sodium   Pyruvate

110.0

55.0

0.5

Thymidine

242.0

0.365

0.0015082645



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