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Product Detail
Basal Medium Eagle (BME)
Price :
$22.00
Catalog Number : B1150-114
Detail
Description
COA
User Guide
Formula
Catalog NumberB1150-114
WithPhenol Red
WithoutSodium Pyruvate,HEPES,L-glutamine
AppearanceClear reddish solution
Product Size500ML
Unit-Price($)22.0
Triple 0.1µm sterile-filtered
Storage conditions2°C to 8°C (protect from light)
Shipping conditionsRoom Temperature
Shelf life12months from date of manufacture


Basal Medium Eagle (BME) is a widely used synthetic basal medium for supporting the growth of many different mammalian cells. BME was originally developed by Harry Eagle for HeLa cells and mouse fibroblasts, when he discovered the minimum requirements for cell growth in vitro. BME has since been used for other human lines, including WI-38 and MRC-5.

BME contains eight B vitamins, the ten essential amino acids, plus cystine, tyrosine, and glutamine. There are a number of modifications of BME: Minimal Essential Medium, Glasgow's Medium and Dulbecco's Modified Eagle's Medium. This BME formulation contains Earle's salts for use in a CO2 incubator.

CAUTION

Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.   






Basal Medium Eagle (BME)    B1150-114

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

L-Arginine   hydrochloride

211.0

21.0

0.09952607

L-Cystine   2HCl

313.0

16.0

0.05111821

L-Histidine

155.0

8.0

0.051612902

L-Isoleucine

131.0

26.0

0.19847329

L-Leucine

131.0

26.0

0.19847329

L-Lysine   hydrochloride

183.0

36.47

0.19928962

L-Methionine

149.0

7.5

0.05033557

L-Phenylalanine

165.0

16.5

0.1

L-Threonine

119.0

24.0

0.20168068

L-Tryptophan

204.0

4.0

0.019607844

L-Tyrosine   disodium salt dihydrate

261.0

26.0

0.099616855

L-Valine

117.0

23.5

0.2008547

Vitamins

Biotin

244.0

1.0

0.0040983604

Choline   chloride

140.0

1.0

0.007142857

D-Calcium   pantothenate

477.0

1.0

0.002096436

Folic   Acid

441.0

1.0

0.0022675737

Nicotinamide

122.0

1.0

0.008196721

Pyridoxal   hydrochloride

204.0

1.0

0.004901961

Riboflavin

376.0

0.1

2.6595744E-4

Thiamine   hydrochloride

337.0

1.0

0.002967359

i-Inositol

180.0

2.0

0.011111111

Inorganic   Salts

Calcium   Chloride (CaCl2) (anhyd.)

111.0

200.0

1.8018018

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0

97.7

0.81416667

Potassium   Chloride (KCl)

75.0

400.0

5.3333335

Sodium   Bicarbonate (NaHCO3)

84.0

2200.0

26.190475

Sodium   Chloride (NaCl)

58.0

6800.0

117.24138

Sodium   Phosphate monobasic (NaH2PO4-H2O)

138.0

140.0

1.0144928

Other   Components

D-Glucose   (Dextrose)

180.0

1000.0

5.5555553

Phenol   Red

376.4

10.0

0.026567481


































































































































































































































































































































































































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