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Product Detail

Medium 199

Price :
$12.30
Catalog Number : B1135-055
Detail
Description
COA
User Guide
Formula
Catalog NumberB1135-055
WithL-glutamine, Phenol Red,L-Amino acids
WithoutHEPES, Sodium Pyruvate
AppearanceClear reddish solution
Product Size500ML
Unit-Price($)12.3
Triple 0.1µm sterile-filtered
Storage conditions2°C to 8°C (protect from light)
Shipping conditionsRoom Temperature
Shelf life 12 months from date of manufacture



Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. BIOEXPLORER® offer a variety of   Medium 199 modifications for a range of cell culture applications. Compared to other basal media, Medium 199 contains unique components, including adenine, adenosine, hypoxanthine, thymine, and additional vitamins. Medium 199 is available with Earle's salts for use in a CO2 incubator, or with Hanks' salts for use without CO2.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.






Medium 199   B1135-055

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

Glycine

75.0

50.0

0.6666667

L-Alanine

89.0

25.0

0.28089887

L-Arginine   hydrochloride

211.0

70.0

0.33175355

L-Aspartic   acid

133.0

30.0

0.22556391

L-Cysteine   hydrochloride-H2O

176.0

0.1

5.681818E-4

L-Cystine   2HCl

240.0

26.0

0.108333334

L-Glutamic   Acid

147.0

75.0

0.5102041

L-Glutamine

146.0

100.0

0.6849315

L-Histidine   hydrochloride-H2O

210.0

21.88

0.10419047

L-Hydroxyproline

131.0

10.0

0.07633588

L-Isoleucine

131.0

40.0

0.3053435

L-Leucine

131.0

60.0

0.45801526

L-Lysine   hydrochloride

183.0

70.0

0.38251367

L-Methionine

149.0

15.0

0.10067114

L-Phenylalanine

165.0

25.0

0.15151516

L-Proline

115.0

40.0

0.3478261

L-Serine

105.0

25.0

0.23809524

L-Threonine

119.0

30.0

0.25210086

L-Tryptophan

204.0

10.0

0.04901961

L-Tyrosine   disodium salt dihydrate

261.0

58.0

0.22222222

L-Valine

117.0

25.0

0.21367522

Vitamins

Ascorbic   Acid

176.0

0.05

2.840909E-4

Biotin

244.0

0.01

4.0983607E-5

Choline   chloride

140.0

0.5

0.0035714286

D-Calcium   pantothenate

477.0

0.01

2.096436E-5

Folic   Acid

441.0

0.01

2.2675737E-5

Menadione   (Vitamin K3)

172.0

0.01

5.8139532E-5

Niacinamide

122.0

0.025

2.0491803E-4

Nicotinic   acid (Niacin)

123.0

0.025

2.0325204E-4

Para-Aminobenzoic   Acid

137.0

0.05

3.6496352E-4

Pyridoxal   hydrochloride

204.0

0.025

1.2254903E-4

Pyridoxine   hydrochloride

206.0

0.025

1.21359226E-4

Riboflavin

376.0

0.01

2.6595744E-5

Thiamine   hydrochloride

337.0

0.01

2.967359E-5

Vitamin   A (acetate)

328.0

0.1

3.0487805E-4

Vitamin   D2 (Calciferol)

397.0

0.1

2.5188917E-4

alpha   Tocopherol phos. Na salt

554.7

0.01

1.8027762E-5

i-Inositol

180.0

0.05

2.7777778E-4

Inorganic   Salts

Calcium   Chloride (CaCl2) (anhyd.)

111.0

200.0

1.8018018

Ferric   nitrate (Fe(NO3)-9H2O)

404.0

0.7

0.001732673

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0

97.67

0.8139166

Potassium   Chloride (KCl)

75.0

400.0

5.3333335

Sodium   Bicarbonate (NaHCO3)

84.0

2200.0

26.190475

Sodium   Chloride (NaCl)

58.0

6800.0

117.24138

Sodium   Phosphate monobasic (NaH2PO4-H2O)

138.0

140.0

1.0144928

Other   Components

2-deoxy-D-ribose

134.0

0.5

0.0037313432

Adenine   sulfate

404.0

10.0

0.024752475

Adenosine   5'-phosphate

347.0

0.2

5.763689E-4

Adenosine   5'-triphosphate

605.0

1.0

0.0016528926

Cholesterol

387.0

0.2

5.1679584E-4

D-Glucose   (Dextrose)

180.0

1000.0

5.5555553

Glutathione   (reduced)

307.0

0.05

1.6286645E-4

Guanine   hydrochloride

188.0

0.3

0.0015957447

Hypoxanthine   Na

136.0

0.4

0.0029411765

Phenol   Red

376.4

20.0

0.053134963

Ribose

150.0

0.5

0.0033333334

Sodium   Acetate

82.0

50.0

0.6097561

Thymine

126.0

0.3

0.0023809525

Tween   80®


20.0

Infinity

Uracil

112.0

0.3

0.0026785715

Xanthine-Na

152.0

0.34

0.0022368422



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