As an improvement on the more used neuronal cell culture additive, the B-27 additive (minus Vitamin A) is a customized version of the original B-27 additive after the removal of Vitamin A (retinol acetate). The B-27 additive (Vitamin A removed) is A serum-free additive that is ideal for the culture of Neural progenitors and Neural stem cells, both in suspended neurospheres and adherent monolayer cultures, without inducing differentiation. B-27 additive (minus vitamin A) is available in 50X liquid form and is suitable for use with Neurobasal medium or Neurobasal-A medium for neuronal cell culture without astrocyte trophoblast.