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Product Detail
DMEM ,High Glucose, no Pyruvate
Price :
$11.30
Catalog Number : B1101-005
Detail
Description
COA
User Guide
Formula
Catalog NumberB1101-005
WithHigh Glucose,L-glutamine,Phenol Red
WithoutHEPES,Sodium Pyruvate
AppearanceClear reddish solution
PH    7.4
Product Size500ML
Unit-Price($)11.3
Triple 0.1µm sterile-filtered
Storage conditions2°C to 8°C (protect from light)
Shipping conditionsRoom Temperature
Shelf life12 months from date of manufacture

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

BIOEXPLORER®offer a variety of DMEM modifications for a range of cell culture applications. DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.






DMEM, high glucose    B1101-005

Components

Molecular Weight

Concentration (mg/L)

Mm

Amino Acids




Glycine

75.0

30.0

0.4

L-Arginine hydrochloride

211.0

84.0

0.39810428

L-Cystine   2HCl

313.0

63.0

0.20127796

L-Glutamine

146.0

584.0

4.0

L-Histidine   hydrochloride-H2O

210.0

42.0

0.2

L-Isoleucine

131.0

105.0

0.8015267

L-Leucine

131.0

105.0

0.8015267

L-Lysine   hydrochloride

183.0

146.0

0.7978142

L-Methionine

149.0

30.0

0.20134228

L-Phenylalanine

165.0

66.0

0.4

L-Serine

105.0

42.0

0.4

L-Threonine

119.0

95.0

0.79831934

L-Tryptophan

204.0

16.0

0.078431375

L-Tyrosine   disodium salt dihydrate

261.0

104.0

0.39846742

L-Valine

117.0

94.0

0.8034188





Vitamins




Choline   chloride

140.0

4.0

0.028571429

D-Calcium   pantothenate

477.0

4.0

0.008385744

Folic   Acid

441.0

4.0

0.009070295

Niacinamide

122.0

4.0

0.032786883

Pyridoxine   hydrochloride

206.0

4.0

0.019417476

Riboflavin   

376.0

0.4

0.0010638298

Thiamine   hydrochloride

337.0

4.0

0.011869436

i-Inositol   

180.0

7.2

0.04





Inorganic Salts




Calcium   Chloride (CaCl2) (anhyd.)

111.0

200.0

1.8018018

Ferric Nitrate   (Fe(NO3)3"9H2O)

404.0

0.1

2.4752476E-4

Magnesium Sulfate (MgSO4)   (anhyd.)

120.0

97.67

0.8139166

Potassium Chloride (KCl)

75.0

400.0

5.3333335

Sodium Bicarbonate (NaHCO3)

84.0

3700.0

44.04762

Sodium Chloride (NaCl)

58.0

6400.0

110.344826

Sodium Phosphate monobasic   (NaH2PO4-H2O)

138.0

125.0

0.9057971





Other Components




D-Glucose   (Dextrose)

180.0

4500.0

25.0

Phenol Red

376.4

15.0

0.039851222



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