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Product Detail

McCoy's5A Medium

Price :
$12.50
Catalog Number : B1136-060
Detail
Description
COA
User Guide
Formula
Catalog NumberB1136-060

With

L-Glutamine,High Glucose,

Bacto-peptone,Phenol Red

WithoutSodium Pyruvate,HEPES
AppearanceClear reddish solution
Product Size500ML
Unit-Price($)12.5
Triple 0.1µm sterile-filtered
Storage conditions2°C to 8°C (protect from light)
Shipping conditionsRoom Temperature
Shelf life12 months from date of manufacture




McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.

Using McCoy's 5A (modified) Medium,Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.






McCoy's 5A (Modified) Medium    B1136-060

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

Glycine

75.0

7.5

0.1

L-Alanine

89.0

13.9

0.15617977

L-Arginine   hydrochloride

211.0

42.1

0.19952606

L-Asparagine

132.0

45.0

0.3409091

L-Aspartic   acid

133.0

19.97

0.15015037

L-Cysteine

121.0

31.5

0.2603306

L-Glutamic   Acid

147.0

22.1

0.15034014

L-Glutamine

146.0

219.2

1.5013698

L-Histidine   hydrochloride-H2O

210.0

20.96

0.09980952

L-Hydroxyproline

131.0

19.7

0.15038168

L-Isoleucine

131.0

39.36

0.300458

L-Leucine

131.0

39.36

0.300458

L-Lysine   hydrochloride

183.0

36.5

0.19945355

L-Methionine

149.0

14.9

0.099999994

L-Phenylalanine

165.0

16.5

0.1

L-Proline

115.0

17.3

0.15043478

L-Serine

105.0

26.3

0.25047618

L-Threonine

119.0

17.9

0.15042016

L-Tryptophan

204.0

3.1

0.0151960775

L-Tyrosine

181.0

18.1

0.1

L-Valine

117.0

17.6

0.15042736

Vitamins

Ascorbic   Acid

176.0

0.5

0.0028409092

Biotin

244.0

0.2

8.1967213E-4

Choline   chloride

140.0

5.0

0.035714287

D-Calcium   pantothenate

477.0

0.2

4.1928721E-4

Folic   Acid

441.0

10.0

0.022675738

Niacinamide

122.0

0.5

0.0040983604

Nicotinic   acid (Niacin)

123.0

0.5

0.0040650405

Para-Aminobenzoic   Acid

137.0

1.0

0.00729927

Pyridoxal   hydrochloride

204.0

0.5

0.0024509805

Pyridoxine   hydrochloride

206.0

0.5

0.0024271845

Riboflavin

376.0

0.2

5.319149E-4

Thiamine   hydrochloride

337.0

0.2

5.934718E-4

Vitamin   B12

1355.0

2.0

0.0014760147

i-Inositol

180.0

36.0

0.2

Inorganic   Salts

Calcium   Chloride (CaCl2) (anhyd.)

111.0

100.0

0.9009009

Magnesium   Sulfate (MgSO4-7H2O)

246.0

200.0

0.8130081

Potassium   Chloride (KCl)

75.0

400.0

5.3333335

Sodium   Bicarbonate (NaHCO3)

84.0

2200.0

26.190475

Sodium   Chloride (NaCl)

58.0

6460.0

111.37931

Sodium   Phosphate monobasic (NaH2PO4-H2O)

138.0

580.0

4.2028985

Other   Components

Bacto-Peptone


600.0

Infinity

D-Glucose   (Dextrose)

180.0

3000.0

16.666666

Glutathione   (reduced)

307.0

0.5

0.0016286644

Phenol   Red

376.4

10.0

0.026567481



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