Ham's F-10 Nutrient Mix--BIOEXPLORER

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Ham's F-10 Nutrient Mix

Price: $11.80Catalog Number: B1278-033

Detail
Description
COA
User Guide
Formula

Catalog Number	B1278-033
With	L-Glutamine,High Glucose,Phenol Red , Sodium Pyruvate
Without	HEPES
Appearance	Clear reddish solution
Product Size	500ML
Unit-Price($）	11.8
Triple 0.1µm sterile-filtered
Storage conditions	2°C to 8°C (protect from light)
Shipping conditions	Room Temperature
Shelf life	12 months from date of manufacture

Compared to other basal media, F-10 contains a wider variety of components, including zinc, hypoxanthine, and thymidine. Serum-free growth of CHO cells in F-10 has led to a variety of improved formulations, such as Ham's F-12 Nutrient Mixture (F-12). F-10 contains no proteins or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). F-10 uses a sodium bicarbonate buffer system (1.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

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Ham's F-10 Nutrient Mix B1278-033
Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75.0	7.5	0.1
L-Alanine	89.0	9.0	0.101123594
L-Arginine hydrochloride	211.0	211.0	1.0
L-Asparagine-H2O	150.0	15.0	0.1
L-Aspartic acid	133.0	13.0	0.09774436
L-Cysteine	121.0	25.0	0.20661157
L-Glutamic Acid	147.0	14.7	0.1
L-Glutamine	146.0	146.0	1
L-Histidine hydrochloride-H2O	210.0	23.0	0.10952381
L-Isoleucine	131.0	2.6	0.019847328
L-Leucine	131.0	13.0	0.099236645
L-Lysine hydrochloride	183.0	29.0	0.15846995
L-Methionine	149.0	4.5	0.030201342
L-Phenylalanine	165.0	5.0	0.030303031
L-Proline	115.0	11.5	0.1
L-Serine	105.0	10.5	0.1
L-Threonine	119.0	3.6	0.0302521
L-Tryptophan	204.0	0.6	0.0029411765
L-Tyrosine disodium salt dihydrate	261.0	2.62	0.010038313
L-Valine	117.0	3.5	0.02991453
Vitamins
Biotin	244.0	0.024	9.836066E-5
Choline chloride	140.0	0.7	0.005
D-Calcium pantothenate	477.0	0.7	0.0014675052
Folic Acid	441.0	1.3	0.0029478457
Niacinamide	122.0	0.6	0.004918033
Pyridoxine hydrochloride	206.0	0.2	9.708738E-4
Riboflavin	376.0	0.4	0.0010638298
Thiamine hydrochloride	337.0	1.0	0.002967359
Vitamin B12	1355.0	1.4	0.0010332103
i-Inositol	180.0	0.5	0.002777778
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111.0	33.3	0.29999998
Cupric sulfate (CuSO4-5H2O)	250.0	0.0025	1.0E-5
Ferric sulfate (FeSO4-7H2O)	278.0	0.834	0.003
Magnesium Sulfate (MgSO4) (anhyd.)	120.0	74.62	0.6218334
Potassium Chloride (KCl)	75.0	285.0	3.8
Potassium Phosphate monobasic (KH2PO4)	136.0	83.0	0.6102941
Sodium Bicarbonate (NaHCO3)	84.0	1200.0	14.285714
Sodium Chloride (NaCl)	58.0	7400.0	127.586205
Sodium Phosphate dibasic (Na2HPO4) anhydrous	142.0	153.7	1.0823944
Zinc sulfate (ZnSO4-7H2O)	288.0	0.03	1.0416666E-4
Other Components
D-Glucose (Dextrose)	180.0	1100.0	6.111111
Hypoxanthine Na	159.0	4.7	0.029559746
Lipoic Acid	206.0	0.2	9.708738E-4
Phenol Red	376.4	1.2	0.0031880978
Sodium Pyruvate	110.0	110.0	1.0
Thymidine	242.0	0.7	0.002892562