Medium 199 ,HEPES--BIOEXPLORER

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Medium 199 ,HEPES

Price: $12.50Catalog Number: B1135-059

Detail
Description
COA
User Guide
Formula

Catalog Number	B1135-059
With	L-glutamine, Phenol Red，HEPES
Without	Sodium Pyruvate
Appearance	Clear reddish solution
Product Size	500ML
PH	7.4
Unit-Price($）	12.5
Triple 0.1µm sterile-filtered
Storage conditions	2°C to 8°C (protect from light)
Shipping conditions	Room Temperature
Shelf life	12 months from date of manufacture

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells. Medium 199 is widely used in virology, vaccine production, and in vitro cultivation of primary explants of mouse pancreatic epithelium, and rat lens tissues. BIOEXPLORER®offer a variety of Medium 199 modifications for a range of cell culture applications. Medium 199 contains no proteins, lipids, or growth factors. Medium 199 uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

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Medium 199, Earle's Salts B1135-059
Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
Glycine	75.0	50.0	0.6666667
L-Alanine	89.0	25.0	0.28089887
L-Arginine hydrochloride	211.0	70.0	0.33175355
L-Aspartic acid	133.0	30.0	0.22556391
L-Cysteine hydrochloride-H2O	176.0	0.1	5.681818E-4
L-Cystine 2HCl	240.0	26.0	0.108333334
L-Glutamic Acid	147.0	75.0	0.5102041
L-Glutamine	146.0	100.0	0.6849315
L-Histidine hydrochloride-H2O	210.0	21.88	0.10419047
L-Hydroxyproline	131.0	10.0	0.07633588
L-Isoleucine	131.0	40.0	0.3053435
L-Leucine	131.0	60.0	0.45801526
L-Lysine hydrochloride	183.0	70.0	0.38251367
L-Methionine	149.0	15.0	0.10067114
L-Phenylalanine	165.0	25.0	0.15151516
L-Proline	115.0	40.0	0.3478261
L-Serine	105.0	25.0	0.23809524
L-Threonine	119.0	30.0	0.25210086
L-Tryptophan	204.0	10.0	0.04901961
L-Tyrosine disodium salt dihydrate	261.0	58.0	0.22222222
L-Valine	117.0	25.0	0.21367522
Vitamins
Ascorbic Acid	176.0	0.05	2.840909E-4
Biotin	244.0	0.01	4.0983607E-5
Choline chloride	140.0	0.5	0.0035714286
D-Calcium pantothenate	477.0	0.01	2.096436E-5
Folic Acid	441.0	0.01	2.2675737E-5
Menadione (Vitamin K3)	172.0	0.01	5.8139532E-5
Niacinamide	122.0	0.025	2.0491803E-4
Nicotinic acid (Niacin)	123.0	0.025	2.0325204E-4
Para-Aminobenzoic Acid	137.0	0.05	3.6496352E-4
Pyridoxal hydrochloride	204.0	0.025	1.2254903E-4
Pyridoxine hydrochloride	206.0	0.025	1.21359226E-4
Riboflavin	376.0	0.01	2.6595744E-5
Thiamine hydrochloride	337.0	0.01	2.967359E-5
Vitamin A (acetate)	328.0	0.1	3.0487805E-4
Vitamin D2 (Calciferol)	397.0	0.1	2.5188917E-4
alpha Tocopherol phos. Na salt	554.7	0.01	1.8027762E-5
i-Inositol	180.0	0.05	2.7777778E-4
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.)	111.0	200.0	1.8018018
Ferric nitrate (Fe(NO3)-9H2O)	404.0	0.7	0.0017326733
Magnesium Sulfate (MgSO4) (anhyd.)	120.0	97.67	0.8139166
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Sodium Bicarbonate (NaHCO3)	84.0	2200.0	26.190475
Sodium Chloride (NaCl)	58.0	6800.0	117.24138
Sodium Phosphate monobasic (NaH2PO4-H2O)	138.0	140.0	1.0144928
Other Components
2-deoxy-D-ribose	134.0	0.5	0.0037313432
Adenine sulfate	404.0	10.0	0.024752475
Adenosine 5'-phosphate	347.0	0.2	5.763689E-4
Adenosine 5'-triphosphate	605.0	1.0	0.0016528926
Cholesterol	387.0	0.2	5.1679584E-4
D-Glucose (Dextrose)	180.0	1000.0	5.5555553
Glutathione (reduced)	307.0	0.05	1.6286645E-4
Guanine hydrochloride	188.0	0.3	0.0015957447
Hypoxanthine Na	136.0	0.4	0.0029411765
Phenol Red	376.4	20.0	0.053134963
Ribose	150.0	0.5	0.0033333334
Sodium Acetate	82.0	50.0	0.6097561
Thymine	126.0	0.3	0.0023809525
Tween 80®		20.0	Infinity
Uracil	112.0	0.3	0.0026785715
Xanthine-Na	152.0	0.34	0.0022368422