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MEM Alpha Modification Medium
Price: $11.30Catalog Number: B1131-053
  • Detail
  • Description
  • COA
  • User Guide
  • Formula
Catalog Number B1131-053
With

Lglutamine,PhenolRed,Ribonucleosides,

Deoxyribonucleosides

Without HEPES
Appearance Clear reddish solution
Product Size 500ML
PH 7.4
Unit-Price($) 11.3
Triple 0.1µm sterile-filtered
Storage conditions 2°C to 8°C (protect from light)
Shipping conditions Room Temperature
Shelf life 12months from date of manufacture











MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. BIOEXPLORER® offer a variety of MEM α modifications for a range of cell culture applications.

CAUTION

Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.





MEM α, nucleosides    B1131-053

Components

Molecular Weight

Concentration (mg/L)

mM

Amino   Acids

Glycine

75.0

50.0

0.6666667

L-Alanine

89.0

25.0

0.28089887

L-Arginine   hydrochloride

211.0

105.0

0.49763033

L-Asparagine-H2O

150.0

50.0

0.33333334

L-Aspartic   acid

133.0

30.0

0.22556391

L-Cysteine   hydrochloride-H2O

176.0

100.0

0.5681818

L-Cystine   2HCl

313.0

31.0

0.09904154

L-Glutamic   Acid

147.0

75.0

0.5102041

L-Glutamine

146.0

292.0

2.0

L-Histidine

155.0

31.0

0.2

L-Isoleucine

131.0

52.4

0.4

L-Leucine

131.0

52.0

0.39694658

L-Lysine

183.0

73.0

0.3989071

L-Methionine

149.0

15.0

0.10067114

L-Phenylalanine

165.0

32.0

0.19393939

L-Proline

115.0

40.0

0.3478261

L-Serine

105.0

25.0

0.23809524

L-Threonine

119.0

48.0

0.40336135

L-Tryptophan

204.0

10.0

0.04901961

L-Tyrosine   disodium salt

225.0

52.0

0.23111111

L-Valine

117.0

46.0

0.3931624

Vitamins

Ascorbic   Acid

176.0

50.0

0.2840909

Biotin

244.0

0.1

4.0983607E-4

Choline   chloride

140.0

1.0

0.007142857

D-Calcium   pantothenate

477.0

1.0

0.002096436

Folic   Acid

441.0

1.0

0.0022675737

Niacinamide

122.0

1.0

0.008196721

Pyridoxal   hydrochloride

204.0

1.0

0.004901961

Riboflavin

376.0

0.1

2.6595744E-4

Thiamine   hydrochloride

337.0

1.0

0.002967359

Vitamin   B12

1355.0

1.36

0.0010036901

i-Inositol

180.0

2.0

0.011111111

Inorganic   Salts

Calcium   Chloride (CaCl2) (anhyd.)

111.0

200.0

1.8018018

Magnesium   Sulfate (MgSO4) (anhyd.)

120.0

97.67

0.8139166

Potassium   Chloride (KCl)

75.0

400.0

5.3333335

Sodium   Bicarbonate (NaHCO3)

84.0

2200.0

26.190475

Sodium   Chloride (NaCl)

58.0

6800.0

117.24138

Sodium   Phosphate monobasic (NaH2PO4-H2O)

138.0

140.0

1.0144928

Ribonucleosides

Adenosine

267.0

10.0

0.037453182

Cytidine

243.0

10.0

0.041152265

Guanosine

283.0

10.0

0.03533569

Uridine

244.0

10.0

0.040983606

Deoxyribonucleosides

2'Deoxyadenosine

251.0

10.0

0.03984064

2'Deoxycytidine   HCl

264.0

11.0

0.041666668

2'Deoxyguanosine

267.0

10.0

0.037453182

Thymidine

242.0

10.0

0.041322313

Other   Components

D-Glucose   (Dextrose)

180.0

1000.0

5.5555553

Lipoic   Acid

206.0

0.2

9.708738E-4

Phenol   Red

376.4

10.0

0.026567481

Sodium   Pyruvate

110.0

110.0

1.0



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